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. 2016 May 4;14(1):129–136. doi: 10.3892/mmr.2016.5208

Figure 1.

Figure 1

PPARG is a direct target gene of miR-128. Neonatal rat ventricular myocytes were transfected with a miR-128 mimic (100 nM), antagomir-128 (100 nM) or miR-control (100 nM) for 48 h. (A) The miR-128 levels were determined by RT-qPCR. (B) RT-qPCR showed that regulation of miR-128 levels affects the mRNA level of PPARG. (C) Representative western blot image of PPARG and ACTB protein levels. (D) Quantitative analyses of the expression of PPARG protein in the western blot analysis. PPARG values were normalized to ACTB expression levels. (E) Transient transfection of HEK293 cells with the PPARG 3′-UTR-luci-WT and PPARG 3′-UTR-luci-MUT constructs, along with the miR-128 mimic, antagomir-128 or miR-control added to the cultures 24 h following transfection. Luciferase activities were measured and normalized to the control luciferase activity. (F) The myocardial miR-128 levels were determined by RT-qPCR. Values are presented as the mean ± standard deviation; n=8 for each group. *P<0.05 vs. the control group or sham group; #P<0.05 vs. the I/R group. PPARG, peroxisome proliferator-activated receptor gamma; miR, microRNA; RT-qPCR, reverser transcription-quantitative polymerase chain reaction; ACTB, β-actin; UTR, untranslated region; luci, luciferase; WT, wild type; MUT, mutant.