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. 2016 May 4;14(1):129–136. doi: 10.3892/mmr.2016.5208

Figure 2.

Figure 2

Determination of myocardial p-Akt, t-Akt, PPARG, Mcl-1 and ACTB protein expression by western blot analysis. (A) Representative western blot images showing p-Akt and t-Akt expression in extracts from the border zone of the myocardium of the (a) sham-operated, (b) myocardial I/R injury, (c) GW9662, (d) antagomir-128 and (e) antagomir-128+GW9662 group. (B) Representative western blot images showing PPARG and Mcl-1 expression in extracts from the border zone of the myocardium of the (a) sham-operated, (b) myocardial I/R injury, (c) GW9662, (d) antagomir-128 and (e) antagomir-128+GW9662 group. Quantification of the relative expression levels of (C) t-Akt, (D) p-Akt, (E) PPARG protein and (F) Mcl-1 protein. The values obtained by densitometric measurements were normalized to ACTB expression levels. Values are presented as the mean ± standard deviation; n=8 for each group. *P<0.05 vs. the sham-operated group; #P<0.05 vs. the I/R group. p-Akt, phosphorylated Akt; t-Akt, total Akt; PPARG, peroxisome proliferator-activated receptor gamma; Mcl-1, myeloid leukemia cell differentiation protein-1; ACTB, β-actin; I/R, ischemia/reperfusion.