Table I.
Gene | Domain | Primers | Accession no. | Annealing temperature (°C) | Expected length (bp) |
---|---|---|---|---|---|
ErbB4 | 5′ terminus | 5′-GCCAAAAATGAAGCTGGCGA-3′ | NM_010154.1 | 60 | 219 |
3′ terminus | 5′-TTGTGCTCGATGCTGGTGAT-3′ | ||||
Neu | 5′ terminus | 5′-TAGCTGGGCATTCACCCTAC-3′ | BC053078.1 | 55 | 358 |
3′ terminus | 5′-AGGGCATAAGCTGTGTCACC-3′ | ||||
Nrg1 | 5′ terminus | 5′-GTCTCAGAGGGTGCCTCAAC-3′ | NM_178591.2 | 60 | 332/230 |
3′ terminus | 5′-GTTCTTCCGGGTGGGTACTG-3′ | ||||
GAPDH | 5′ terminus | 5′-GTGGAGTCATACTGGAACATGTAG-3′ | 60 | 150 | |
3′ terminus | 5′-AATGGTGAAGGTCGGTGTG-3′ |
Nrg1, neuregulin 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.