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. 2016 May 23;14(1):790–796. doi: 10.3892/mmr.2016.5325

Table I.

Sequences of primers for reverse transcription-polymerase chain reaction.

Gene Domain Primers Accession no. Annealing temperature (°C) Expected length (bp)
ErbB4 5′ terminus 5′-GCCAAAAATGAAGCTGGCGA-3′ NM_010154.1 60 219
3′ terminus 5′-TTGTGCTCGATGCTGGTGAT-3′
Neu 5′ terminus 5′-TAGCTGGGCATTCACCCTAC-3′ BC053078.1 55 358
3′ terminus 5′-AGGGCATAAGCTGTGTCACC-3′
Nrg1 5′ terminus 5′-GTCTCAGAGGGTGCCTCAAC-3′ NM_178591.2 60 332/230
3′ terminus 5′-GTTCTTCCGGGTGGGTACTG-3′
GAPDH 5′ terminus 5′-GTGGAGTCATACTGGAACATGTAG-3′ 60 150
3′ terminus 5′-AATGGTGAAGGTCGGTGTG-3′

Nrg1, neuregulin 1; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.