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. 2016 May 13;14(1):195–201. doi: 10.3892/mmr.2016.5283

Figure 4.

Figure 4

Induction of A375 cell apoptosis after indirect co-culture with TRAIL-ADSCs. (A) A375 cells were cocultured with ADSCs or TRAIL-ADSCs in a Transwell system, with A375 cells as control. After 24 or 48 h culture, the cells were stained with PI. Red staining indicates dead cells. Phase 1, whole population of cells that still attached to the culture surface assessed under the phase contrast microscope; PI, apoptotic cells stained with PI show red; merge, merged images of Phase 1 and PI. Original magnification, ×100. (B) Fluorescence-activated flow cytometry was used to detect A375 cell apoptosis after staining with PI in co-culture with TRAIL-ADSCs or ADSCs at a different ratio in the Transwell system. rTRAIL served as a positive control and A375 cells alone served as a negative control. Ratio indicates the cell number of A375 cells to ADSCs or TRAIL-ADSCs. (C) Anti-human TRAIL antibody diluted at different concentrations was used to neutralize the TRAIL-induced apoptosis, which was shown in co-culture of A375 cells and TRAIL-ADSCs at a 1:1 ratio. *P<0.05 vs. control, #P<0.05 vs. rhTRAIL. TRAIL, tumor necrosis factor-related apoptosis-inducing ligand; ADSCs, adipose-derived stem cells.