Fig. 3. FAO (slow freezing) and BeWo (rapid freezing) cryopreservation. (A) FAO cell metabolic activity post-freeze–thaw as determined by resazurin assay; (B) BeWo cell metabolic activity post-freeze–thaw as determined by resazurin assay; (C) β-hCG secretion (pg mL^–1) due to forskolin induced BeWo cell syncytialization, post cryopreservation. All data from at least 3 repeats and errors bars represent ± standard deviation. * represents p ≤ 0.05 relative to DMSO only.