Fig. 4. Rat hepatocyte cytotoxicity and cryopreservation (slow freezing). (A) Resazurin fluorescence (cell metabolic activity assay) over 2 hours in rat hepatocytes with and without of 20 mg mL–1 PVA; (B) cell metabolic activity (%) of cryopreserved rat hepatocytes (1 × 106 cells per mL) each plated overnight, relative to 10% (v/v) DMSO preservation alone as judged by the resazurin assay after 6 hours. Freezing in complete medium alone gave 0% cell recovery; all data from at least 3 repeats and errors bars represent ± standard deviation. * represents p ≤ 0.05 relative to DMSO only.
