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. Author manuscript; available in PMC: 2016 Jun 23.
Published in final edited form as: Clin Sci (Lond). 2016 Mar 18;130(11):931–941. doi: 10.1042/CS20160076

Figure 5. miR-143 overexpression reduces expression of glycolytic enzymes and rescues mitochondrial function in trophoblast cells of A2GDM.

Figure 5

(A) Representative immunoblots and (B) quantification of glycolytic enzyme expression in trophoblast cells of CTRL and of A2GDM with and without overexpression of miR-143, n = 4. (C) Representative immunoblots and (D) quantification of mitochondrial complex expression in trophoblast cells of CTRL and of A2GDM with or without overexpression of miR-143. n = 3 for complex I and n = 4 for complexes II–V, Values are mean ± S.E.M. *P<0.05 compared with CTRL, aP<0.05 compared with A2GDM. Mitochondrial respiratory parameters were measured in ST of (E) CTRL and (F) A2GDM pregnancies with or without miR-143. OCR were measured using a Seahorse Bioscience XF24 extracellular flux analyser as described previously [21]. (G) OCR values for each respiration parameter in A2GDM with or without miR-143 were normalized to values in CTRL cells. n = 4 placentas in each group. Values are mean ± S.E.M. *P< 0.05 compared with CTRL, aP<0.05 compared with A2GDM.