Fig. 2.

Enzymatic activity of BAAT mutants. BAAT-specific N-acyltransferase and thioesterase activities were measured simultaneously for purified recombinant BAAT forms, wild-type, BAAT-S, BAAT-SKL, BAAT-Ex1Del, and BAAT-12AA Trunc, via MS. A: Thioesterase activity (striped bars) was measured by the accumulation of free cholic acid while N-acyltransferase activity was measured by the production of taurocholate (gray bars). B: Specific activity values are expressed as a percentage of wild-type enzyme. Error bars represent the standard error of the mean as calculated for four separate identical experiments.