Fig. 3.

Deletion of the hepatic Lpl gene decreases plasma LPL contents and activity. The control and mutant adult mice were pretreated with heparin, and then their plasma was harvested for measuring LPL contents and activity. A, B: Plasma LPL contents were determined by ELISA (A) and Western blot (B). Plasma (15 μl) from paired mice was resolved by SDS-PAGE gel before probing with anti-LPL antibody. C: Plasma LPL activity was decreased by the deletion of hepatic Lpl. Results represent mean ± SEM. *P < 0.05 versus control; n = 4–5 for each group.