Fig. 1.

Atgl deficiency has differential effect on neutral lipids in mouse HSCs. HSCs isolated from WT or Atgl^−/− mice were incubated for 1, 7, or 14 days in normal medium. Subsequently, neutral lipids were determined by HPLC-MS. Values of all TAG species, TAG (A), CE (B), and RE (C), were expressed relative to the amount of cholesterol in the same sample. D–F: The relative net loss or gain of all TAG species (D), specific TAG species without a PUFA chain (E), and specific TAG species with one or more PUFA-chains (F) during the incubation as values are expressed relative to the level of the respective lipids present at day 1. Data are the mean ± SEM of six mice. *P < 0.05 ANOVA, WT versus Atgl^−/−.