Fig. 2.

Atgl-deficient mouse HSCs have a lower relative breakdown of newly labeled TAG species. A, B: Primary WT and ATGL^−/− mouse HSCs were incubated on day 1 with D4-palmitate and D8-arachidonic acid for 2 days. At day 3, part of the cells were harvested (label) and the remaining HSCs were chased for 48 h with normal medium and harvested at day 5 (chase). Subsequently, neutral lipids were extracted and HPLC-MS was performed as described. A: Single or double deuterium-labeled (gray and dark gray bars) and nonlabeled (white bars) TAG fragments with two palmitoyl chains (16:0, 16:0, x) and a palmitoyl and an oleoyl chain (16:0, 18:1, x) were quantitated and expressed as percentage of all TAG species. B: Single, double, or triple deuterium-labeled (gray, dark gray, and black bars) and nonlabeled (white bars) forms of di-arachidonoyl-palmitoyl glycerol (20:4, 20:4, 16:0) were quantitated and expressed as percentage of all TAG species. C: Shows breakdown of the labeled TAG species as the data from the indicated TAG fragments of the cells at day 5, after the chase, were expressed relative to the level of the same TAG species at the beginning of the chase at day 3. Data are the mean ± SEM of five experiments performed in duplicate. *P < 0.05 t-test versus WT.