Fig. 6.

High turnover of TAG in rat HSCs. A, B: Primary rat HSCs were incubated on day 1 with D4-palmitate for 2 days. At day 3, some of the cells were harvested (label) and the remaining HSCs were chased for 24 h with normal medium and harvested at day 4 (chase). Subsequently, neutral lipids were extracted and HPLC-MS was performed as described. A: Single or double deuterium-labeled (gray and dark gray bars) and nonlabeled (white bars) TAG fragments with two palmitoyl chains (16:0, 16:0, x) and a palmitoyl and an oleoyl chain (16:0, 18:1, x) were quantitated and expressed as percentage of all TAG species. B: Shows breakdown of the labeled TAG species as the data from the indicated TAG fragments of the cells at day 4, after the chase, were expressed relative to the level of the same TAG species at the beginning of the chase at day 3. Data are the mean ± SD of three experiments performed in duplicate.