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. 2016 Jul;57(7):1162–1174. doi: 10.1194/jlr.M066415

Fig. 7.

Fig. 7.

Effect of Atglistatin and the DGAT1 inhibitor, T863, on TAG metabolism in rat HSCs. A, B: Isolated rat HSCs were either labeled on day 6 with D4-palmitate for 1 day in the presence or absence of 20 μM T863 (label 1d) or labeled for 2 days from day 1 to day 3 (label 2d) and subsequently chased for another 2 days in the presence of vehicle (DMSO; chase cont) or 50 μM Atglistatin (chase + Atglst). Neutral lipids were determined by HPLC-MS. A: Single or double deuterium-labeled (gray and dark gray bars) and nonlabeled (white bars) TAG fragments with two palmitoyl chains (16:0, 16:0, x) were quantitated and expressed as percentage of all TAG species. B: Shows breakdown of the D4-palmitate-labeled TAG species as the data from the cells after the chase, were expressed relative to the level of the same TAG species at the beginning of the chase. C, D: Isolated rat HSCs were incubated from day 1 to day 7 with vehicle (DMSO; control), 50 μM Atglistatin (Atglst) (C), or 20 μM T863 (D). Subsequently, neutral lipids were determined by HPLC-MS. The values were normalized to the amount of cholesterol in the sample and expressed relative to the level of the respective lipids present in the control cells at day 7. Data are the mean ± SD of three experiments performed in duplicate. *P < 0.05 t-test versus control.