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. 2016 Jul;57(7):1243–1255. doi: 10.1194/jlr.M067397

Fig. 6.

Fig. 6.

AMPK played an important role in the inhibition of ER stress and lipolysis by curcumin. Adipose tissues were incubated with curcumin for 4 h. A: AMPK phosphorylation was determined by Western blot. B: Adipose tissue was incubated with curcumin followed by stimulation with PA for 24 h; IRE1α and eIF2α phosphorylation was determined by Western blot. C: The 3T3-L1 cells were transfected with AMPKα1/α2 or control siRNAs; expression of p-IRE1α and p-eIF2α was determined by Western blot. D: The p65 phosphorylation in adipose tissue exposed to thapsigargin (Thaps) stimulation for 24 h was detected by Western blot. E: PDE3B expression in adipocytes subjected to Thaps challenge when AMPKα1/α2 or control siRNAs were silenced with siRNAs. F: The 3T3-L1 cells were transfected with AMPKα1/α2 or control siRNAs, content of IL-6 in the medium was detected by ELISA kit (n = 6) and CM was used to incubate hepatocytes for 24 h. G: Insulin-mediated Akt phosphorylation in hepatocytes was detected by Western blot. The results are expressed as the mean ± SD of three independent experiments. *P < 0.05 versus model; #P < 0.05 versus indicated treatment.