Table 1. Contribution of coa, vwb and clfA to structure and development of the abscess.
| Strain | WT | ΔclfA | Δcoa | Δvwb | Δcoa/Δvwb | PBS ctrl | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Days post-infection | d2 | d6 | d10 | d2 | d6 | d10 | d2 | d6 | d10 | d2 | d6 | d10 | d2 | d6 | d10 | d6 | d10 |
| Capsule granulation tissue | 0/2 | 2/2 | 2/2 | 0/2 | 2/2 | 2/2 | 0/2 | 2/2 | 0/2 | 0/2 | 2/2 | 2/2 | 0/2 | 1/2 | 1/2 | 0/2 | 0/2 |
| Fibrous capsule | 0/2 | 0/2 | 2/2 | 0/2 | 2/2 | 2/2 | 0/2 | 0/2 | 2/2 | 0/2 | 0/2 | 2/2 | 0/2 | 0/2 | 2/2 | 0/2 | 0/2 |
| Epithelialization | 0/2 | 2/2 | 1/2 | 0/2 | 0/2 | 1/2 | 0/2 | 1/2 | 0/2 | 0/2 | 1/2 | 0/2 | 0/2 | 0/2 | 1/2 | 0/2 | 0/2 |
| Vasculitis/vascular necrosis | 2/2 | 2/2 | 2/2 | 1/2 | 0/2 | 0/2 | 2/2 | 2/2 | 0/2 | 2/2 | 2/2 | 1/2 | 2/2 | 2/2 | 0/2 | 0/2 | 0/2 |
| Thrombosis | 2/2 | 2/2 | 1/2 | 0/2 | 0/2 | 0/2 | 2/2 | 2/2 | 0/2 | 1/2 | 2/2 | 0/2 | 2/2 | 2/2 | 0/2 | 0/2 | 0/2 |
| Coagulative necrosis | 2/2 | 2/2 | 1/2 | 0/2 | 0/2 | 2/2 | 2/2 | 1/2 | 1/2 | 1/2 | 2/2 | 0/2 | 0/2 | 2/2 | 0/2 | 0/2 | 0/2 |
| Extracellular bacteria | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 0/2 | 0/2 |
| Intracellular bacteria | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 1/2 | 1/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 2/2 | 0/2 | 0/2 |
| Compiled score | 10/16 | 14/16 | 13/16 | 5/16 | 8/16 | 11/16 | 9/16 | 11/16 | 7/16 | 8/16 | 13/16 | 9/16 | 8/16 | 11/16 | 8/16 | 0/16 | 0/16 |
Abscesses were scored based on the presence (1) or absence (0) of a chosen feature based on 6 histopathology sections of two abscesses (3 each) for each bacterial strain. 0/2 = present in neither abscess; 1/2 = present in 1 of 2 abscesses; 2/2 = present in both abscesses.