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. 2016 Jun 14;11:2839–2854. doi: 10.2147/IJN.S104505

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© 2016 Gan et al. This work is published and licensed by Dove Medical Press Limited

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PI staining detection of injured Vero cells after exposure to nano-COM and COD crystals.

Notes: PI staining detection by fluorescence microscope (A) and relative fluorescence intensity by microplate reader (B) of injured Vero cells after exposure to 100 μg/mL nano-COM or COD crystals for 6 hours. The injured Vero cells were obtained by different concentrations of H₂O₂ treatment (0, 0.3, 0.5, 1.0 mmol/L). Data were expressed as mean ± standard deviation from three independent experiments. Magnification: ×600.

Abbreviations: COM, calcium oxalate monohydrate; COD, calcium oxalate dihydrate; PI, propidium iodide.