Table 1. Fitted parameters for α4β2, α4β2V287L, α5α4β2, and α5α4β2V287L ACh concentration-response relations in oocytes.
| Receptor | α:β mRNA Ratio | Component | % of Total | EC50 (μM) |
|---|---|---|---|---|
| α4β2 | 1:1 | HSa | 65 ± 2 (8)b | 0.67 ± 0.08 (8) |
| LS | 35 ± 2 (8) | 190 ± 60 (8) | ||
| α4β2V287L | 1:1 | HS | 100c | 0.11 ± 0.01 (8)*** |
| α4β2 | 10:1 | HS | 7 ± 4 (17) | 0.67 (fixed)d |
| LS | 93 ± 4 (17) | 190 (fixed) | ||
| α4β2V287L | 10:1 | HS | 23 ± 3 (7)**e | 0.11 (fixed) |
| LS | 77 ± 3 (7) | 36 ± 7 (7)* | ||
| α5α4β2 | 10:1:1 | HS | 100 | 0.26 ± 0.04 (4) |
| α5α4β2V287L | 10:1:1 | HS | 100 | 0.32 ± 0.01 (8) |
aHS and LS denote the high- and low-sensitivity components of the concentration-response relations, respectively.
bValues are mean ± S.E. (number of oocytes).
cFor single-component concentration-response relations the percent of total (% of total) was fixed at 100%.
dParameters constrained to a particular value are denoted as (fixed).
eMutant values that differ significantly from WT (α4β2) at the 0.05. 0.01, and 0.001 levels are marked (*), (**), and (***).