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. 2016 Jun 23;11(6):e0157992. doi: 10.1371/journal.pone.0157992

Fig 6. Nedd9 is critical for osteoclast differentiation.

Fig 6

(A) Realtime PCR analysis of effects of retroviral overexpression of Nedd9 gene on RANKL-induced osteoclastogenesis, as indicated by expression of Nedd9 and Cathepsin K mRNAs. (B) Effects of SB431542 (SB) treatment on osteoclastogenesis, as evaluated by TRAP staining, in Nedd9-overexpressing cells treated with RANKL. Overexpression of Nedd9 increased RANKL-induced osteoclastogenesis. SB431542 suppressed osteoclastogenesis, which was partly recovered by Nedd9 overexpression. Cultures were stained by TRAP. Bars = 100 μm. (C) The expression of Nedd9 gene as determined by realtime PCR. (D) The number of TRAP positive osteoclasts was significantly increased by Nedd9 overexpression, and the suppression of osteoclastogenesis by SB431542 was partly suppressed by Nedd9 overexpression. *P < 0.05.