Fig 2. Effect of miRNA overexpression on target expression.

MiR-141-3p and/or miR-145-5p were overexpressed in renal cancer cell lines 786-O and ACHN for 48 h. Effect on mRNA expression of putative targets, which were predicted to be regulated by miR-141-3p and miR-145-5p, was determined by RT-qPCR. Data are shown as mean (± SEM) relative to NC#1. NC#1 = negative control, n = 3, student’s t-test (two-tailed); *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns = not significant.