Reagent | Type | Manufacturer | Catalog # | Comments |
---|---|---|---|---|
Olaparib | Inhibitor | Selleck Chemicals | S1060 | Source shared during communication with authors. |
DMSO | Chemical | Sigma Aldrich | 472301 | Source shared during communication with authors. |
Phosphate buffered saline (PBS) | Buffer | Gibco-Life Technologies | 10010-023 | Source shared during communication with authors. |
Giemsa stain | Chemical | Sigma Aldrich | G5637 | Source shared during communication with authors. |
Methanol | Chemical | Fisher Scientific | BP1105-4 | Source shared during communication with authors. |
DoTc2-4510 cells | Cell line | ATCC | CRL-7920 | Original source not specified. |
MES-SA cells | Cell line | ATCC | CRL-1976 | Original source not specified |
U-2-OS cells | Cell line | ATCC | HTB-96 | Original source not specified. |
SAOS-2 cells | Cell line | ATCC | HTB-85 | Original source not specified. |
SJSA-1 cells | Cell line | ATCC | CRL-2098 | Original source not specified. |
HOS cells | Cell line | ATCC | CRL-1543 | Original source not specified. |
MG-63 cells | Cell line | ATCC | CRL-1427 | Original source not specified. |
143B cells | Cell line | ATCC | CRL-8303 | Replaces osteosarcoma cells used originally; see Known Differences. |
G-292 cells, clone A141B1 | Cell line | ATCC | CRL-1423 | |
A673 cells | Cell line | ATCC | CRL-1598 | Replaces the ES cells used originally; see Known Differences |
SK-N-MC cells | Cell line | ATCC | HTB-10 | |
TC-71 cells2 | Cell line | Children’s Oncology Group Cell Culture and Xenograft Repository |
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CHLA-10 cells1 | Cell line | Children’s Oncology Group Cell Culture and Xenograft Repository |
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CHLA-9 cells3 | Cell line | Children’s Oncology Group Cell Culture and Xenograft Repository |
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Iscove’s modified DMEM (IMDM) | Cell culture | Life Technologies | 12440-053 | Not originally included. |
L-glutamine | Cell culture | Life Technologies | 25030-081 | Not originally included. |
Insulin-Transferrin-Selenium (ITS) | Growth factor | Lonza | 17-838Z | Not originally included. |
McCoy’s 5A Medium Modified | Cell culture | ATCC | 30-2007 | Not originally included. |
Fetal bovine serum (FBS) | Cell culture | Valley Biomedical | BS3032 | Original source not specified. |
RPMI 1640 medium | Cell culture | ATCC | 30-2001 | Original source not specified. |
Eagle’s Minimum Essential Media (EMEM) |
Cell culture | ATCC | 30-2003 | Originally not specified. |
5-bromo-2’-deoxyuridine | Nucleoside | Sigma | B5002 | Not originally included. |
MEM Eagle with Earle’s BSS | Cell culture | Lonza | 12-125F | Not originally included. |
DMEM – High Glucose | Cell culture | GE-Healthcare | E15-883 | Shared during communication with authors. |
DMEM/F12 | Cell culture | Life Technologies | 11320-033 | Original source not specified. |