Reagent | Type | Manufacturer | Catalog # | Comments |
---|---|---|---|---|
EWS-FLI1 transformed mouse mesenchymal progenitor cells (MPCs) |
Cell line | Authors | N/A | Provided by the Stamenkovic lab |
FUS-CHOP transformed mouse mesenchymal progenitor cells (MPCs) |
Cell line | Authors | N/A | Provided by the Stamenkovic lab |
SK-N-MC cells | Cell line | ATCC | HTB-10 | Source shared during communication with authors. |
Olaparib | Inhibitor | Selleck Chemicals | S1060 | Source shared during communication with authors. |
DMSO | Chemical | Sigma | D8418 | Source shared during communication with authors. |
4% formaldehyde | Chemical | USB | 19943 | Source shared during communication with authors. |
Syto60 fluorescent nucleic acid stain | Chemical | Invitrogen | S11342 | Catalog # shared during communication with authors. |
FBS | Cell culture | Valley Biomedical | BS3032 | Original source not specified. |
RPMI 1640 medium | Cell culture | ATCC | 30-2001 | Original source not specified. |
Fluorescent plate reader | Equipment | LiCor | Source shared during communication with authors. |
|
DMEM, low glucose, GlutaMAX supplement, pyruvate |
Cell culture | Gibco | 21885-025 | Shared during communication with authors. |
MCDB 201 medium, with trace elements, L-glutamine and 30 mM HEPES; powder |
Cell culture | Sigma | M6770 | Shared during communication with authors. |
Ascorbic acid-2-phosphate | Cell culture | Sigma | A8960 | Shared during communication with authors. |
Dexamethasone | Chemical | Sigma | D8893 | Shared during communication with authors. |
Linoleic acid-BSA | Chemical | Sigma | L9530 | Shared during communication with authors. |
Insulin, transferrin, sodium selenite supplement |
Growth factor | Roche (Sigma) | 1074547 | Shared during communication with authors. |
Dialyzed FCS | Cell culture | Sigma | F0392 | Shared during communication with authors. |
EGF; human | Growth factor | Sigma | E9644 | Shared during communication with authors. |
PDGF-BB, rat | Growth factor | R&D Systems | 520-BB-050 | Shared during communication with authors. |
Penicillin-Streptomycin; 100X | Cell culture | Sigma | P4333 | Original source not specified. |
Leukemia inhibitory factor (LIF); human; 10 µg/ml |
Growth factor | Sigma | L5283 | Shared during communication with authors. Replaces LIF generated from CHO LIF720D cells. |
Fibronectin; 0.1% in PBS | Chemical | Sigma | F1141 | Shared during communication with authors. |