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. 2016 Jun 23;5:e13620. doi: 10.7554/eLife.13620
Reagent Type Manufacturer Catalog # Comments
EWS-FLI1 transformed mouse
mesenchymal progenitor cells (MPCs)
Cell line Authors N/A Provided by the Stamenkovic lab
FUS-CHOP transformed mouse
mesenchymal progenitor cells (MPCs)
Cell line Authors N/A Provided by the Stamenkovic lab
SK-N-MC cells Cell line ATCC HTB-10 Source shared during
communication with authors.
Olaparib Inhibitor Selleck Chemicals S1060 Source shared during
communication with authors.
DMSO Chemical Sigma D8418 Source shared during
communication with authors.
4% formaldehyde Chemical USB 19943 Source shared during
communication with authors.
Syto60 fluorescent nucleic acid stain Chemical Invitrogen S11342 Catalog # shared during
communication with authors.
FBS Cell culture Valley Biomedical BS3032 Original source not specified.
RPMI 1640 medium Cell culture ATCC 30-2001 Original source not specified.
Fluorescent plate reader Equipment LiCor Source shared during
communication with authors.
DMEM, low glucose,
GlutaMAX supplement, pyruvate
Cell culture Gibco 21885-025 Shared during
communication with authors.
MCDB 201 medium, with trace elements,
L-glutamine and 30 mM HEPES; powder
Cell culture Sigma M6770 Shared during
communication with authors.
Ascorbic acid-2-phosphate Cell culture Sigma A8960 Shared during
communication with authors.
Dexamethasone Chemical Sigma D8893 Shared during
communication with authors.
Linoleic acid-BSA Chemical Sigma L9530 Shared during
communication with authors.
Insulin, transferrin,
sodium selenite supplement
Growth factor Roche (Sigma) 1074547 Shared during
communication with authors.
Dialyzed FCS Cell culture Sigma F0392 Shared during
communication with authors.
EGF; human Growth factor Sigma E9644 Shared during
communication with authors.
PDGF-BB, rat Growth factor R&D Systems 520-BB-050 Shared during
communication with authors.
Penicillin-Streptomycin; 100X Cell culture Sigma P4333 Original source not specified.
Leukemia inhibitory factor (LIF);
human; 10 µg/ml
Growth factor Sigma L5283 Shared during communication with authors.
Replaces LIF generated from CHO LIF720D cells.
Fibronectin; 0.1% in PBS Chemical Sigma F1141 Shared during
communication with authors.