Table 1.

Full factorial design for xylanase production (XY) in solid-state fermentation (SSF) by Aspergillus japonicus URM5620 under different operational conditions after 120 h of cultivation

Run	Sa (g)a	Im (%)b	pHc	T (°C)d	XY
					(U g ds−1)e	(U mg−1)f
1	5.0	15	4.0	25	8200	29,491
2	5.0	15	4.0	35	401	1810
3	5.0	35	4.0	25	8967	24,128
4	5.0	35	4.0	35	8898	24,378
5	10.0	15	4.0	25	4217	19,027
6	10.0	15	4.0	35	5517	21,130
7	10.0	35	4.0	25	4455	12,367
8	10.0	35	4.0	35	2659	7880
9	5.0	15	6.0	25	29,085	62,141
10	5.0	15	6.0	35	2766	6256
11	5.0	35	6.0	25	7664	17,032
12	5.0	35	6.0	35	3585	8883
13	10.0	15	6.0	25	4188	11,243
14	10.0	15	6.0	35	7452	20,573
15	10.0	35	6.0	25	4137	9540
16	10.0	35	6.0	35	3075	7901
17	7.5	25	5.0	30	5661	16,162
18	7.5	25	5.0	30	7479	22,819
19	7.5	25	5.0	30	6723	17,590
20	7.5	25	5.0	30	6387	17,381

^a Sa-substrate amount (g, grams), ^b Im-initial moisture (%), ^c pH and ^d T-temperature (°C), ^e U gds⁻¹—xylanase activity, ^f U mg⁻¹—specific xylanase activity