Table 2.
Effects of PEG molar mass (M PEG), PEG concentration (C PEG), citrate concentration (C C) and pH on partition coefficient (K), top activity yield (Y), purification factor in the top phase (PF), selectivity (S) using 24 experimental design to xylanase extraction by PEG/citrate ATPS
| Run | M aPEG | C bPEG | Ccc | pH | K d | Y e | PFf | Sg |
|---|---|---|---|---|---|---|---|---|
| A1 | 1000 | 20 | 15 | 6.0 | 27.20 | 268.01 | 7.16 | 185.25 |
| A2 | 8000 | 20 | 15 | 6.0 | 9.66 | 183.03 | 6.05 | 83.04 |
| A3 | 1000 | 24 | 15 | 6.0 | 35.98 | 265.12 | 5.36 | 208.14 |
| A4 | 8000 | 24 | 15 | 6.0 | 19.28 | 220.00 | 4.00 | 95.91 |
| A5 | 1000 | 20 | 20 | 6.0 | 47.15 | 192.73 | 3.27 | 146.06 |
| A6 | 8000 | 20 | 20 | 6.0 | 31.41 | 163.59 | 1.13 | 5.52 |
| A7 | 1000 | 24 | 20 | 6.0 | 38.49 | 184.92 | 3.66 | 138.71 |
| A8 | 8000 | 24 | 20 | 6.0 | 44.44 | 179.17 | 4.88 | 293.33 |
| A9 | 1000 | 20 | 15 | 8.0 | 45.27 | 229.94 | 6.51 | 286.69 |
| A10 | 8000 | 20 | 15 | 8.0 | 24.39 | 198.47 | 6.68 | 218.78 |
| A11 | 1000 | 24 | 15 | 8.0 | 28.68 | 205.94 | 3.38 | 105.20 |
| A12 | 8000 | 24 | 15 | 8.0 | 20.62 | 192.35 | 2.44 | 67.42 |
| A13 | 1000 | 20 | 20 | 8.0 | 37.87 | 160.77 | 3.94 | 135.58 |
| A14 | 8000 | 20 | 20 | 8.0 | 28.10 | 181.54 | 4.65 | 128.86 |
| A15 | 1000 | 24 | 20 | 8.0 | 30.23 | 154.14 | 2.05 | 67.48 |
| A16 | 8000 | 24 | 20 | 8.0 | 50.78 | 207.08 | 2.50 | 108.60 |
| A17 | 3350 | 22 | 17.5 | 7.0 | 24.31 | 207.10 | 7.20 | 203.35 |
| A18 | 3350 | 22 | 17.5 | 7.0 | 21.56 | 184.27 | 4.91 | 141.84 |
| A19 | 3350 | 22 | 17.5 | 7.0 | 22.86 | 199.04 | 5.58 | 152.38 |
| A20 | 3350 | 22 | 17.5 | 7.0 | 28.96 | 239.34 | 5.81 | 231.20 |
aPEG molar mass (g mol−1), b PEG concentration (%), c citrate concentration (%), d partition coefficient, e top activity yield (%), f purification factor in the top phase, g selectivity