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. Author manuscript; available in PMC: 2017 Jul 7.
Published in final edited form as: Chem Commun (Camb). 2016 Jun 10;52(53):8267–8270. doi: 10.1039/c6cc02894b

Fig. 3.

Fig. 3

Comparison of in-solution click reactions and Dicer cleavage. (a) General scheme and table of click reactions. - = no pre-miRNA. (b) pre-miRNA IEDDA products detected via fluorescence gel. Click reactions were performed with 2.0 equiv. of L-Y for 2 h at 25 °C. Top = Detection of rhodamine–pre-miRNA conjugate. Rhodamine fluorescence was first detected, followed by addition of SYBR GOLD for 5 min and RNA detection on the same gel. Middle = Detection of HRP–pre-miRNA conjugate using SYBR GOLD. Bottom = Overexposure and zoom of the band representing the HRP–pre-miRNA conjugate. (c) pre-miRNA maturation catalyzed by Dicer. Lane 1 = miRNA ladder; Lane 2 = pre-miRNA-TCO; Lane 3 = pre-miRNA-TCO + Dicer; Lane 4 = pre-miRNA-mTet + Dicer; Lane 5 = pre-miRNA-mTet.