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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 May 26;36(7):1434–1447. doi: 10.1161/ATVBAHA.116.307573

Figure 3. iPSDMs recapitulate AS events in M1 activation.

Figure 3

A, RT-PCR validation of bioinformatically identified differential AS events in HMDMs and iPSDMs. % exon inclusion and SD calculated from gel densitometry of (inclusion PCR product) / (total PCR product). B, Correlation plot of AS events predicted for each gene (calculated to be value θ) in M1-HMDM vs. M1-iPSDM. Spearman's rho = 0.77, P < 0.001. C, Correlation plot of differential expression of splicing factors on the log(2) scale. Spearman's rho = 0.78, P < 0.001. iPSDMs = induced pluripotent stem cell derived macrophages; RT-PCR = reverse transcriptase polymerase chain reaction; SD = standard deviation; AS = alternative splicing; HMDM = human monocyte derived macrophage.