Table 2.
Homologies of differentially expressed cDNA-AFLP fragments with known gene sequences in database using BLASTN algorithm along their expression patterns in excess B-treated C. grandis (CG) and Citrus sinensis (CS) roots.
| TDF # | Size (bp) | Homologies | Organism origin | E-value | Similarity (%) | Sequence coverage (%) | Genebank ID | Ratio of BE/CK | |
|---|---|---|---|---|---|---|---|---|---|
| CG | CS | ||||||||
| CARBOHYDRATE AND ENERGY METABOLISM | |||||||||
| 232_1 | 286 | Cytochrome c oxidase subunit Vb | Dimocarpus longan | 7.00E-37 | 92 | 46 | AEQ61828.1 | 0 | |
| 101_5 | 179 | NADH dehydrogenase subunit 4, partial (mitochondrion) | Actinidia polygama | 1.00E-26 | 96 | 9 | CAD60445.1 | 0 | |
| 41_2 | 315 | Electron transfer flavoprotein-ubiquinone oxidoreductase | Medicago truncatula | 2.00E-51 | 80 | 17 | XP_003597528.1 | 0.41 | |
| 98_2 | 254 | Pyruvate dehydrogenase E1 alpha subunit | Arabidopsis thaliana | 1.00E-26 | 83 | 16 | NP_171617.1 | 0.32 | |
| 53_4 | 251 | Pyruvate kinase | Arabidopsis thaliana | 5.00E-39 | 96 | 12 | NP_001078275.1 | 0 | 0.35 |
| 185_1 | 241 | Phosphoglycerate kinase | Arabidopsis thaliana | 3.00E-15 | 51 | 14 | NP_178073.1 | 0 | |
| 150_1 | 391 | Alternative oxidase | Mangifera indica | 7.00E-33 | 87 | 21 | CAA55892.1 | 0 | |
| 137_2 | 204 | Cytochrome P450 | Arabidopsis thaliana | 2.00E-19 | 66 | 11 | AAB67854.1 | 0.29 | |
| 9_2 | 201 | Ferredoxin-NADP reductase, root isozyme 2 | Arabidopsis thaliana | 8.00E-30 | 86 | 15 | NP_973942.1 | 2.25 | |
| 26_2 | 314 | Dephospho-CoA kinase | Medicago truncatula | 5.00E-44 | 73 | 26 | XP_003625901.1 | 2.83 | |
| 248_1 | 261 | Sucrose synthase | Citrus unshiu | 2.00E-44 | 100 | 7 | BAA89049.1 | 0.27 | |
| 116_2 | 353 | NADP-dependent D-sorbitol-6-phosphate dehydrogenase | Medicago truncatula | 2.00E-39 | 86 | 27 | XP_003607080.1 | 2.43 | |
| CELL WALL AND CYTOSKELETON METABOLISM | |||||||||
| 65_1 | 305 | Root hair defective 3 GTP-binding protein (RHD3) | Arabidopsis thaliana | 4.00E-25 | 84 | 13 | NP_177439.2 | + | |
| 151_2 | 303 | Alpha-1,4-glucan-protein synthase [UDP-forming], putative | Ricinus communis | 3.00E-60 | 92 | 30 | XP_002525910.1 | 0.34 | |
| 37_2 | 240 | Fucosyltransferase 8 | Arabidopsis thaliana | 2.00E-21 | 63 | 15 | NP_001077531.1 | 0 | |
| 99_2 | 254 | Polygalacturonase-like protein | Arabidopsis thaliana | 3.00E-11 | 54 | 17 | BAA95779.1 | 0 | |
| 50_1 | 255 | Villin 4 | Arabidopsis thaliana | 2.00E-17 | 71 | 6 | NP_194745.1 | 0 | |
| 26_1 | 385 | O-methyltransferase | Citrus sinensis x Citrus reticulata | 1.00E-41 | 65 | 36 | ABP94018.1 | 0 | |
| 231_3 | 219 | Beta-D-xylosidase 4 | Arabidopsis thaliana | 7.00E-29 | 87 | 9 | NP_201262.1 | 0.46 | |
| 138_1 | 415 | Cell wall-associated hydrolase, partial | Medicago truncatula | 5.00E-44 | 96 | 19 | XP_003637074.1 | + | |
| 249_1 | 384 | Protein SPIRAL1-like2 | Arabidopsis thaliana | 3.00E-27 | 80 | 62 | NP_177083.1 | 3.72 | |
| 253_1 | 346 | S-acyltransferase | Arabidopsis lyrata subsp.lyrata | 5.00E-39 | 76 | 15 | XP_002887235.1 | 0.51 | |
| LIPID METABOLISM | |||||||||
| 183_2 | 380 | Glycerol-3-phosphate acyltransferase 9 | Arabidopsis thaliana | 9.00E-62 | 82 | 28 | NP_568925.1 | 0.29 | |
| 44_1 | 95 | Dihydroxyacetone kinase | Arabidopsis thaliana | 4.00E-05 | 84 | 4 | NP_188404.1 | + | |
| 249_2 | 330 | Coproporphyrinogen III oxidase | Arabidopsis thaliana | 3.00E-55 | 88 | 23 | NP_171847.4 | 0.33 | |
| 24_2 | 328 | Cinnamate 4-hydroxylase | Citrus x paradisi | 7.00E-27 | 100 | 19 | AAK57011.1 | 0.41 | |
| 53_3 | 284 | Lipoxygenase | Vitis vinifera | 9.00E-41 | 80 | 10 | ACZ17393.1 | 0 | |
| 129_3 | 290 | Fatty acid/sphingolipid desaturase | Arabidopsis thaliana | 3.00E-40 | 80 | 17 | NP_182144.1 | 0 | 0 |
| 150_2 | 379 | Acyl carrier protein | Camellia oleifera | 2.00E-16 | 87 | 53 | AFK31314.1 | 0 | |
| 89_2 | 295 | Acyl carrier protein 3 | Helianthus annuus | 1.00E-30 | 81 | 70 | ADV16367.1 | 0 | |
| 9_1 | 213 | Citrus dioxygenase | Citrus limetta | 6.00E-36 | 94 | 20 | AER36089.1 | + | |
| 42_1 | 261 | 1,4-dihydroxy-2-naphthoyl-CoA thioesterase 1 | Arabidopsis thaliana | 2.00E-31 | 81 | 32 | NP_175266.1 | 3.61 | |
| 103_6 | 152 | JAZ3 | Vitis rupestris | 2.00E-11 | 70 | 13 | AEP60134.1 | 0.24 | |
| NUCLEIC ACID METABOLISM | |||||||||
| 140_2 | 249 | Histone deacetylase 19 | Arabidopsis thaliana | 6.00E-30 | 81 | 13 | NP_001078511.1 | 2.7 | |
| 129_1 | 299 | Histone H4 | Arabidopsis thaliana | 1.00E-32 | 100 | 45 | NP_001077477.1 | 2.2 | |
| 148_1 | 208 | Zinc finger protein CONSTANS-LIKE 5-like | Glycine max | 5.00E-25 | 87 | 18 | NP_001239972.1 | + | |
| 82_1 | 213 | DNA binding protein | Arabidopsis lyrata subsp. Lyrata | 2.00E-04 | 74 | 5 | XP_002889547.1 | 0.5 | |
| 28_1 | 308 | PREDICTED: ATP-dependent DNA helicase recG-like | Vitis vinifera | 6.00E-15 | 53 | 10 | XP_002280664.2 | 0 | |
| 131_4 | 296 | Transcription initiation factor IIB-2 | Arabidopsis thaliana | 2.00E-51 | 93 | 23 | NP_187644.1 | 0.36 | |
| 60_1 | 395 | Poly(A)-binding protein | Daucus carota | 5.00E-24 | 80 | 16 | AAK30205.1 | 0 | |
| 246_1 | 309 | Transcription factor TCP15 | Arabidopsis thaliana | 1.00E-41 | 89 | 19 | NP_564973.1 | 0 | 0 |
| 79_1 | 272 | KNAT4 homeobox protein | Arabidopsis thaliana | 4.00E-47 | 99 | 18 | CAA63131.1 | + | |
| 123_2 | 287 | DEAD-box ATP-dependent RNA helicase 20 | Arabidopsis thaliana | 5.00E-30 | 77 | 16 | NP_175911.1 | 4.1 | |
| 195_1 | 268 | Ribonucleotide reductase RNR1 like protein, partial | Arabidopsis thaliana | 1.00E-46 | 94 | 27 | CAA69026.1 | 0 | |
| 133_4 | 273 | Putative phosphoribosylaminoimidazole carboxylase/AIR carboxylase | Arabidopsis thaliana | 1.00E-40 | 80 | 11 | NP_181305.2 | 0 | |
| 134_1 | 308 | Polypyrimidine tract-binding protein 1 | Arabidopsis thaliana | 9.00E-53 | 90 | 20 | NP_186764.1 | 0 | |
| 142_1 | 231 | Transcription factor/ transcription initiation factor | Medicago truncatula | 7.00E-24 | 74 | 13 | XP_003597404.1 | 0.53 | |
| 252_1 | 310 | AT1G56110, partial | Arabidopsis thaliana | 5.00E-24 | 74 | 23 | BAH20197.1 | 0.33 | |
| 81_3 | 325 | Hypothetical protein POPTR_0001s24860g | Populus trichocarpa | 2.00E-49 | 79 | 14 | XP_002299866.1 | 1.84 | |
| PROTEIN AND ACID METABOLISM | |||||||||
| 85_2 | 356 | Ubiquitin-protein ligase 1 | Arabidopsis thaliana | 4.00E-59 | 88% | 3% | AAF36454.1 | 0.38 | |
| 132_3 | 384 | E3 ubiquitin-protein ligase MARCH3 | Medicago truncatula | 6.00E-09 | 86 | 27 | XP_003613768.1 | 0 | 0 |
| 73_2 | 324 | E3 ubiquitin-protein ligase ATL6 | Arabidopsis thaliana | 8.00E-17 | 50 | 23 | AAD33584.1 | 0 | 0 |
| 42_3 | 180 | Ubiquitin, partial | Oryza sativa | 2.00E-26 | 96 | 46 | AAL77200.1 | + | |
| 86_1 | 398 | Ubiquitin-protein ligase, putative | Ricinus communis | 8.00E-25 | 57 | 18 | XP_002520193.1 | 0.43 | |
| 112_1 | 461 | E3 ubiquitin-protein ligase UPL5 | Medicago truncatula | 3.00E-42 | 70 | 18 | XP_003594229.1 | 0 | |
| 27_1 | 333 | ARM repeat superfamily protein | Arabidopsis thaliana | 3.00E-52 | 83 | 6 | NP_198149.2 | 0 | |
| 230_1 | 258 | Aspartic protease, partial | Dimocarpus longan | 1.00E-05 | 71 | 26 | AEJ76922.1 | + | |
| 62_1 | 326 | Aspartic proteinase nepenthesin-1 | Medicago truncatula | 1.00E-37 | 77 | 19 | XP_003627883.1 | 0 | 0 |
| 61_1 | 335 | Protein ASPARTIC PROTEASE IN GUARD CELL 1 | Arabidopsis thaliana | 3.00E-40 | 65 | 18 | NP_188478.1 | 1.79 | |
| 118_5 | 208 | Serine carboxypeptidase II-3 | Medicago truncatula | 3.00E-22 | 72 | 14 | XP_003592243.1 | 0.21 | |
| 41_1 | 141 | Drought-inducible cysteine proteinase RD19A precursor | Arabidopsis thaliana | 5.00E-16 | 86 | 35 | BAD94010.1 | 0 | |
| 24_1 | 352 | Gamma-glutamyl hydrolase 2 | Arabidopsis thaliana | 2.00E-34 | 74 | 25 | NP_565186.2 | 0.35 | |
| 253_4 | 264 | Protein disulfide isomerase | Zea mays | 7.00E-23 | 64 | 13 | ACG47473.1 | 0.43 | |
| 184_1 | 267 | Protein disulfide isomerase-like 1-1 | Arabidopsis thaliana | 2.00E-09 | 56 | 17 | NP_849696.1 | 0.38 | |
| 12_1 | 361 | Elongation factor 1-alpha, partial | Arabidopsis thaliana | 2.00E-21 | 93 | 60 | BAD94755.1 | 2.04 | |
| 121_2 | 160 | 40S ribosomal protein S8 | Zea mays | 3.00E-19 | 98 | 19 | NP_001105391.1 | 2.52 | |
| 48_1 | 303 | 40S ribosomal protein S4 | Medicago truncatula | 2.00E-52 | 90 | 24 | XP_003604568.1 | 0.44 | |
| 246_5 | 228 | 40S ribosomal protein S2 | Arabidopsis lyrata subsp. Lyrata | 5.00E-29 | 87 | 27 | XP_002878157.1 | 0 | |
| 9_3 | 198 | 60S ribosomal protein L7a | Zea mays | 2.00E-15 | 91 | 19 | ACG47588.1 | + | + |
| 153_2 | 261 | 40S ribosomal protein S5 | Capsicum annuum | 4.00E-44 | 100 | 31 | AAR89617.1 | 0 | 0.39 |
| 80_2 | 320 | 50S ribosomal protein L2, chloroplastic | Citrus sinensis | 3.00E-63 | 99 | 62 | YP_740517.1 | 0 | 0 |
| 64_2 | 291 | 60S ribosomal protein L10-1 | Arabidopsis thaliana | 2.00E-54 | 90 | 30 | NP_563945.2 | + | |
| 140_1 | 335 | 40S ribosomal protein S29 | Zea mays | 9.00E-26 | 86 | 74 | ACG30830.1 | 3.12 | |
| 38_1 | 290 | 60S ribosomal protein L15-1 | Arabidopsis thaliana | 2.00E-47 | 84 | 33 | NP_193405.1 | 0.41 | |
| 14_2 | 225 | Translation initiation factor eIF-4A1 | Arabidopsis thaliana | 1.00E-37 | 100 | 13 | CAC43286.1 | 0 | |
| 229_1 | 327 | Methionine synthase 2 | Arabidopsis thaliana | 3.00E-59 | 92 | 12 | NP_187028.1 | 0 | 0.46 |
| 144_2 | 266 | Threonyl-tRNA synthetase | Medicago truncatula | 2.00E-41 | 88 | 11 | XP_003601575.1 | 0 | 0.22 |
| 5_1 | 354 | S-adenosyl-L-homocystein hydrolase | Gossypium hirsutum | 2.00E-27 | 89 | 19 | ACJ11250.1 | 0 | 0 |
| 229_2 | 192 | Bifunctional aminoacyl-tRNA synthetase, putative, expressed | Oryza sativa Japonica Group | 5.00E-30 | 96 | 10 | ABA97740.1 | 0.34 | |
| STRESS RESPONSE | |||||||||
| 182_3 | 214 | Homogentisate phytyltransferase 1 | Arabidopsis thaliana | 3.00E-14 | 51 | 12 | NP_849984.1 | + | + |
| 64_1 | 309 | Obg-like ATPase 1 | Arabidopsis thaliana | 5.00E-51 | 87 | 20 | NP_174346.1 | 0 | |
| 99_3 | 238 | Peroxidase | Medicago truncatula | 3.00E-22 | 60 | 14 | XP_003615990.1 | 0 | |
| 231_5 | 188 | Catalase, partial | Citrus maxima | 9.00E-31 | 91 | 19 | ACY30463.1 | 0 | 0 |
| 30_1 | 320 | DJ-1 family protein | Arabidopsis lyrata subsp.lyrata | 9.00E-28 | 73 | 24 | XP_002894433.1 | 0.24 | |
| 233_5 | 141 | Heat shock protein 70 | Nicotiana benthamiana | 3.00E-05 | 75 | 33 | BAD02271.1 | + | |
| 237_1 | 305 | BAG family molecular chaperone regulator 7 | Arabidopsis thaliana | 4.00E-14 | 67 | 16 | NP_201045.1 | 0.35 | |
| 131_1 | 332 | Putative senescence-associated protein, partial | Trichosanthes dioica | 5.00E-05 | 100 | 100 | ABN50029.1 | + | |
| 17_1 | 296 | Putative senescence-associated protein, partial | Ipomoea ni | 6.00E-52 | 93 | 25 | BAF46313.1 | 2.52 | |
| 104_3 | 171 | WD repeat phosphoinositide-interacting-like protein | Medicago truncatula | 4.00E-21 | 96 | 11 | XP_003591137.1 | 3.15 | |
| 77_5 | 112 | Universal stress protein A-like protein | Medicago truncatula | 2.00E-09 | 74 | 12 | XP_003616191.1 | 0.37 | |
| 140_3 | 220 | ACR toxin-sensitivity inducing protein (mitochondrion) | Citrus jambhiri | 4.00E-10 | 96 | 10 | BAB85481.1 | + | |
| 251_5 | 266 | Ankyrin-like protein | Arabidopsis thaliana | 7.00E-38 | 82 | 9 | BAB10271.1 | 0 | |
| 146_5 | 285 | Leucine-rich repeat containing protein, putative | Ricinus communis | 1.00E-20 | 59 | 8 | XP_002523984.1 | 0 | |
| SIGNAL TRANSDUCTION | |||||||||
| 35_1 | 341 | ATP/GTP/Ca++ binding protein | Cucumis melo subsp.melo | 2.00E-44 | 74 | 18 | ABR67417.1 | 3.04 | |
| 80_3 | 194 | Calcium-dependent lipid-binding domain-containing protein | Arabidopsis thaliana | 2.00E-19 | 80 | 13 | NP_564576.1 | 0.41 | |
| 67_2 | 232 | Calcium-binding EF-hand-containing protein | Arabidopsis thaliana | 2.00E-07 | 52 | 5 | NP_173582.2 | 0.49 | |
| 35_2 | 267 | Calcium ion binding protein, putative | Ricinus communis | 7.00E-32 | 67 | 18 | XP_002534034.1 | 0.48 | |
| 103_2 | 207 | Serine/threonine-protein kinase | Nicotiana attenuata | 3.00E-20 | 71 | 8 | AEI84329.1 | + | |
| 251_3 | 301 | Leucine-rich repeat receptor-like protein kinase | Medicago truncatula | 4.00E-34 | 68 | 15 | XP_003600547.1 | 0 | 0 |
| 53_2 | 350 | ATP binding/kinase/protein serine/threonine kinase | Vitis vinifera | 3.00E-50 | 80 | 12 | CAQ58615.1 | + | |
| 122_1 | 343 | Receptor-like protein kinase-like protein | Glycine max | 4.00E-46 | 77 | 13 | ACM89521.1 | 0 | |
| 103_3 | 207 | 14-3-3 protein | Litchi chinensis | 6.00E-33 | 95 | 19 | ADP00759.1 | + | |
| 117_1 | 184 | Auxin-responsive protein | Gossypium hirsutum | 1.00E-07 | 54 | 15 | AEE25651.1 | 0 | |
| 39_1 | 330 | Phytochrome A, partial | Populus tremula | 2.00E-51 | 86 | 7 | AEK26583.1 | 0 | |
| TRANSPORT | |||||||||
| 251_2 | 316 | H+-ATPase 4, plasma membrane-type | Arabidopsis thaliana | 3.00E-23 | 92 | 10 | NP_190378.2 | + | + |
| 76_3 | 270 | Exocyst complex component 84B | Arabidopsis thaliana | 8.00E-25 | 64 | 9 | NP_199794.1 | 0 | 0 |
| 77_8 | 448 | Vacuolar membrane ATPase subunit c” | Citrus limon | 1.00E-71 | 99 | 50 | AAO73433.1 | 0 | |
| 151_1 | 336 | Exocyst subunit exo70 family protein B1 | Arabidopsis thaliana | 1.00E-50 | 82 | 15 | NP_200651.1 | 0 | |
| 135_1 | 326 | ADP ribosylation factor | Medicago truncatula | 2.00E-50 | 100 | 88 | XP_003591354.1 | 0.49 | |
| 246_4 | 228 | Peroxin 7 | Arabidopsis thaliana | 5.00E-07 | 75 | 16 | NP_174220.1 | 0.45 | |
| 39_2 | 216 | Protein transport protein SEC61 gamma subunit | Medicago truncatula | 3.00E-04 | 90 | 64 | XP_003616767.1 | 0 | |
| 183_3 | 236 | ABC transporter I family member 20 | Arabidopsis thaliana | 1.00E-12 | 69 | 20 | NP_195847.1 | 0.42 | |
| 253_2 | 318 | Metal tolerance protein | Carica papaya | 7.00E-52 | 89 | 26 | ADI24923.1 | 0 | |
| 19_1 | 316 | Sec61 transport protein | Populus trichocarpa | 6.00E-54 | 88 | 16 | XP_002331716.1 | 0.5 | |
| 141_2 | 272 | Synaptobrevin-like protein | Medicago truncatula | 6.00E-35 | 83 | 20 | XP_003617238.1 | 0 | |
| 79_3 | 196 | PRA1 (prenylated RAB acceptor) family protein | Medicago truncatula | 1.00E-18 | 65 | 18 | XP_003597623.1 | 0.31 | |
| 10_1 | 147 | Protein tolB | Medicago truncatula | 1.00E-09 | 72 | 7 | XP_003637684.1 | 0.37 | |
| OTHERS | |||||||||
| 98_3 | 331 | Rubber elongation factor protein | Arabidopsis thaliana | 2.00E-23 | 63 | 31 | NP_187201.1 | + | |
| 133_5 | 259 | FRIGIDA-like protein | Arabidopsis thaliana | 9.00E-25 | 69 | 12 | NP_850923.1 | 0 | |
| 121_1 | 248 | Limonoid UDP-glucosyltransferase | Citrus maxima | 5.00E-11 | 70 | 16 | ABY27084.1 | 0 | 0 |
| 77_4 | 318 | ATP sulfurylase 1 | Arabidopsis thaliana | 2.00E-54 | 97 | 18 | NP_188929.1 | 0 | |
| 38_2 | 134 | FAD-binding berberine family protein | Medicago truncatula | 2.00E-13 | 85 | 6 | XP_003594602.1 | 0.41 | |
| 189_1 | 309 | Probable methyltransferase PMT14 | Arabidopsis thaliana | 4.00E-37 | 62 | 15 | BAH19630.1 | + | |
| 41_3 | 398 | 1-deoxy-D-xylulose-5-phosphate synthase, partial | Bixa orellana | 2.00E-65 | 87 | 68 | AAU29063.1 | 1.9 | |
| 173_1 | 286 | DPP6 N-terminal domain-like protein | Arabidopsis thaliana | 4.00E-21 | 70 | 12 | NP_564147.1 | 0.3 | |
| 66_1 | 284 | F22G5.28 | Arabidopsis thaliana | 6.00E-36 | 68 | 12 | AAF79556.1 | + | |
| 147_4 | 254 | PREDICTED: uncharacterized protein LOC100264520 | Vitis vinifera | 3.00E-38 | 84 | 24 | XP_002276822.1 | 0.51 | |
| 109_3 | 277 | Unnamed protein product, partial | Vitis vinifera | 1.00E-19 | 64 | 50 | CBI31118.3 | 0.28 | |
| 153_3 | 226 | Predicted protein | Arabidopsis lyrata subsp. | 2.00E-16 | 69 | 46 | XP_002871569.1 | 1.78 | |
| 22_4 | 287 | Hypothetical protein VITISV_014691 | Vitis vinifera | 2.00E-27 | 65 | 21 | CAN71367.1 | 2.32 | |
| 158_1 | 177 | PREDICTED: uncharacterized protein LOC100255693 | Vitis vinifera | 5.00E-09 | 51 | 13 | XP_002279857.1 | 1.33 | |
| 21_1 | 231 | AT5G10200-like protein | Arabidopsis arenosa | 1.00E-31 | 90 | 9 | ACK44505.1 | + | |
| 163_3 | 228 | PREDICTED: uncharacterized protein LOC100262433 | Vitis vinifera | 2.00E-30 | 77 | 11 | XP_002284886.1 | 0 | |
| 27_2 | 267 | Predicted protein | Populus trichocarpa | 5.00E-27 | 63 | 30 | XP_002330640.1 | 0 | |
| 116_1 | 401 | Hypothetical protein MTR_1g005920 | Medicago truncatula | 3.00E-25 | 79 | 75 | XP_003588318.1 | 0.39 | |
| 163_2 | 330 | Unnamed protein product | Vitis vinifera | 8.00E-07 | 69 | 6 | CBI37596.3 | + | 0 |
| 170_2 | 212 | Predicted protein | Populus trichocarpa | 7.00E-07 | 68 | 14 | XP_002309501.1 | 0 | |
| 77_6 | 230 | Hypothetical protein MTR_5g051130 | Medicago truncatula | 4.00E-11 | 79 | 6 | XP_003614394.1 | 0.51 | |
| 105_2 | 277 | PREDICTED: UPF0197 transmembrane protein C11orf10 | Glycine max | 3.00E-25 | 87 | 42 | XP_003549239.1 | 2.13 | |
| 108_2 | 144 | Hypothetical protein SORBIDRAFT_0351s002020 | Sorghum bicolor | 7.00E-16 | 100 | 28 | XP_002489033.1 | + | 0 |
| 109_5 | 178 | Unknown | Glycine max | 6.00E-15 | 89 | 57 | ACU14517.1 | + | |
| 118_3 | 287 | Hypothetical protein MTR_5g051140 | Medicago truncatula | 1.00E-14 | 93 | 8 | XP_003614395.1 | 2.47 | |
| 170_1 | 296 | Unknown | Glycine max | 5.00E-31 | 95 | 30 | ACU24256.1 | 1.8 | 2.11 |
| 173_3 | 213 | Hypothetical protein MTR_5g051120 | Medicago truncatula | 1.00E-25 | 88 | 18 | XP_003614393.1 | 1.73 | |
| 178_1 | 306 | Hypothetical protein | Lilium longiflorum | 2.00E-25 | 96 | 100 | ABO20854.1 | + | 0 |
| 193_3 | 244 | Hypothetical protein MTR_4g091430 | Medicago truncatula | 3.00E-11 | 57 | 48 | XP_003608262.1 | + | |
| 228_1 | 306 | Hypothetical protein | Arabidopsis thaliana | 1.00E-39 | 100 | 11 | BAF01964.1 | 1.32 | + |
| 237_3 | 204 | PREDICTED: uncharacterized protein LOC100800109 | Glycine max | 2.00E-31 | 92 | 54 | XP_003541224.1 | + | |
| 249_5 | 224 | Hypothetical protein | Zea mays | 4.00E-20 | 98 | 24 | ACG27665.1 | 1.38 | |
| 37_3 | 217 | Hypothetical protein SORBIDRAFT_0070s002020 | Sorghum bicolor | 3.00E-23 | 82 | 72 | XP_002489102.1 | 3.01 | 0.39 |
| 53_5 | 165 | Hypothetical protein SORBIDRAFT_1994s002010 | Sorghum bicolor | 2.00E-10 | 100 | 28 | XP_002488913.1 | 2.73 | |
| 6_3 | 218 | Hypothetical protein MTR_5g051030 | Medicago truncatula | 8.00E-25 | 95 | 9 | XP_003614386.1 | 2.06 | |
| 85_1 | 369 | Hypothetical protein SORBIDRAFT_1368s002010 | Sorghum bicolor | 7.00E-52 | 91 | 66 | XP_002488947.1 | 0.47 | |
| 94_1 | 222 | Hypothetical protein MTR_8g040260 | Medicago truncatula | 2.00E-06 | 51 | 31 | XP_003627937.1 | + | |
| 146_4 | 245 | Putative uncharacterized protein Sb0351s002020 | Sorghum bicolor | 3.00E-12 | 97 | 47 | XP_002489033.1 | 0.54 | |
| 252_4 | 190 | Conserved hypothetical protein | Ricinus communis | 2.00E-09 | 83 | 19 | XP_002532022.1 | 0.38 | |
| 133_7 | 217 | Hypothetical protein SORBIDRAFT_0227s002010 | Sorghum bicolor | 2.00E-15 | 97 | 48 | XP_002489051.1 | + | 0.41 |
Data are means of three replicates; Expression ratio, + indicates that TDFs were only isolated from excess B-treated roots. #, Number; BE, B-excess; CK, Control. Functional classification was performed based on the information reported for each sequence by The Gene Ontology (http://amigo1.geneontology.org/cgi-bin/amigo/go.cgi) and Uniprot (http://www.uniprot.org/). Ratio of BE/CK was obtained by gel image analysis performed with PDQuest version 8.0.1 (Bio-Rad, Hercules, CA, USA). B-excess-responsive TDFs shared by the two citrus species were highlighted in bold.