Figure 3.

(A) Flip assays for C₆NBD-PC, C₆NBD-PE, and C₆NBD-PS in LUVs without peptides (open circles), containing 0.1 mol % (for C₆NBD-PC), or 0.025 mol % (for C₆NBD-PE and -PS) EDEM1 (red circles), or containing 0.1 mol % ATF6 (blue circles), FACL4 (yellow circles), JP3 (light blue circles), JP4 (purple circles), ORP5 (blue triangles), P4HTM (red triangles), RNF180 (yellow triangles), SPAST (green triangles), TMED7 (light blue triangles), or UBE2J2 (purple triangles). The lines are fitting curves generated using Eq. 2. (B) Flip rate constants of C₆NBD-PC, C₆NBD-PE, and C₆NBD-PS in LUVs containing 0–0.1 mol % peptides. The symbols are the same as in (A). The error bars represent the mean ± SD from n = 2 experiments. To see this figure in color, go online.