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In vitro phosphorylation of FACT and PAF-C by CKII. A, autoradiograph of a representative set of in vitro reactions performed with [γ32P]ATP in the presence or absence of Ctr9-FLAG, Spt16-TAP, or recombinant CKII, as indicated at the top. The location of each phosphorylated form of the FACT, PAF-C, and CKII subunits is indicated at the left and right. B, in vitro kinase reactions for PAF-C phosphorylation by Spt16-associated CKII. Lane numbers are given below each panel for clarity.
