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. 2016 May 3;291(26):13410–13420. doi: 10.1074/jbc.M116.727735

FIGURE 6.

FIGURE 6.

CKII regulates histone H2B-K123ub1 levels. A, Western blotting analysis of histone H2B-K123ub1, total histone H2B, and histone H3 lysine 4 trimethylation levels from whole cell extracts from cka1Δ cka2–8 strains grown under permissive (30 °C) and restrictive (25 °C and 37 °C) temperatures. The antibody used for each panel is indicated at the right, and the temperature used for cell growth is given at the bottom. B, Western blotting analysis of whole cell lysates for histone H2B-K123ub1, G6PDH, H2B, and HA of PAF1 or CTR9 deletion strains rescued with PAF1-3HA or CTR9-3HA expression vectors as indicated. Left panel, Vector, pRS313–3HA-SSN6; PAF1-WT, PAF1–3HA; S/T>A, PAF1–3HA S147A, T385A, T422A, S426A; S/T>E, PAF1–3HA S147E, T385E, T422E, S426E. Right panel, Vector, pRS313–3HA-SSN6; CTR9-WT, CTR9–3HA); S>A, CTR9–3HA S977A, S1015A, S1017A, S1046A, S1054A, S1056A; S>E, CTR9–3HA S977E, S1015E, S1017E, S1046E, S1054E, S1056E.