FIGURE 1.

Characterization of the initiation complex assembled on human HSP promoter. a, schematic illustration of the footprinting data on HSP. Black numbers indicate positions of the bases in promoter DNA relative to the start site (+1), and gray numbers indicate the positions according to the reference (Cambridge) mtDNA. The −11 base in the template DNA stand that interacts with mtRNAP is highlighted in yellow. b, Y162A TFAM mutant is defective in run-off transcription assays on both mitochondrial promoters. nt, nucleotides. c, TFAM variants Y162A and L6 are deficient in transcription on both LSP (black bars) and HSP (red bars). Error bars indicate means ± S.E. d, TFAM variants deficient in transcription on LSP are also defective on HSP.