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Comparison of the binding affinity of the EHD1 EH domain and EHD3 EH domain for a MICAL-L1 NPF peptide by isothermal titration calorimetry. Solutions containing 90 μm purified EH domains of EHD1 or EHD3 were injected with 930 μm MICAL-L1 peptide, and ITC binding isotherms were collected. KD values obtained by fitting the titration curves are indicated for each EH domain.
