FIGURE 10.

Effect of fasting and refeeding on expression of mRNAs encoding components of the Scap-SREBP pathway in livers of Tg-HMGCR (TM1–8) and hmgcr knock-in mice. Total RNA from livers of mice used in Fig. 9, A and D (4 mice/group) was separately isolated. Equal amounts of RNA from individual mice were subjected to quantitative real-time RT-PCR using primers against the indicated gene; apoB mRNA was used as an invariant control. Each value represents the amount of mRNA relative to that in control, nonfasted mice, which was arbitrarily set as 1. Bars, mean ± S.E. (error bars) of data from four mice. PEPCK, phosphoenolpyruvate carboxykinase.