TABLE 7.
Metabolic parameters of WT and hmgcr knock-in mice subjected to fasting and refeeding
Male mice (7–8 weeks of age) were subjected to fasting and refeeding as described under “Experimental Procedures.” WT mice were littermates of hmgcrWT/Ki and hmgcrKi/Ki mice. Each value represents the mean ± S.E. of four mice.
| WT |
hmgcrKi/Ki |
|||||
|---|---|---|---|---|---|---|
| Nonfasted | Fasted | Refed | Nonfasted | Fasted | Refed | |
| Liver cholesterol (mg/g) | 2.1 ± 0.1 | 3.5 ± 0.2 | 2.2 ± 0.1 | 2.5 ± 0.1 | 3.9 ± 0.1 | 2.5 ± 0.1 |
| Liver triglyceride (mg/g) | 5.2 ± 2.1 | 33.1 ± 6.9 | 6.1 ± 0.6 | 3.7 ± 0.6 | 50.4 ± 3.2 | 5.1 ± 0.8 |
| Plasma fatty acids (meq/liter) | 0.64 ± 0.05 | 1.65 ± 0.30 | 0.40 ± 0.04 | 0.64 ± 0.01 | 1.76 ± 0.21 | 0.43 ± 0.08 |
| Plasma triglycerides (mg/dl) | 182 ± 17 | 279 ± 27 | 206 ± 25 | 186 ± 10 | 211 ± 10 | 172 ± 19 |
| Plasma cholesterol (mg/dl) | 127 ± 13 | 145 ± 11 | 87 ± 6 | 134 ± 12 | 144 ± 9 | 116 ± 9 |
| Plasma glucose (mg/dl) | 113 ± 16 | 45 ± 11 | 83 ± 13 | 102 ± 7 | 44 ± 4 | 75 ± 9 |
| Plasma insulin (ng/ml) | 0.70 ± 0.14 | 0.16 ± 0.03 | 1.82 ± 0.63 | 0.50 ± 0.90 | 0.21 ± 0.05 | 1.01 ± 0.26 |
| Plasma insulin (ng/ml) | 2.35 ± 0.15 | 0.24 ± 0.04 | 2.74 ± 0.96 | 1.39 ± 0.12 | 0.24 ± 0.02 | 5.48 ± 0.48 |