FIGURE 1.

Alignment of the PRDM7(195–392) construct studied in this article and the PRDM9(195–385) construct described by Eram et al. (14). A, the PR domains of human PRDM7 (Q9NQW5) and PRDM9 (Q9NQV7) were defined per the Uniprot annotation (gray box, residues 244–358 for both proteins). The three divergent residues in the PR domain between the two sequences are indicated. The start of the C-terminal divergence between the constructs is noted by the arrow at position 368. B, domain architecture of the canonical isoform of PRDM7 (isoform A) and a secondary isoform of PRDM7 (isoform B) that contain 0 or 4 zinc fingers, respectively. PRDM9 is also shown for reference. Arrows indicate the approximate construct boundaries of the PRDM7 (isoform A) recombinant protein used for enzymatic assays. Proteins are represented by SMART protein annotation.