FIGURE 2.

Wild-type PRDM7 is an active methyltransferase. A, PRDM7 is thermally stabilized by AdoMet (dashed line) relative to vehicle control (solid line), as shown by differential scanning fluorimetry. B, AdoMet (SAM) stabilizes PRDM7 in a concentration-dependent manner. C, PRDM7 is a trimethylase that is selective for H3K4me2. Peptide screening was performed with 1 μm wild-type PRDM7 and 5 μm test peptide as described under “Experimental Procedures.” Data in panel A are presented as representative raw traces. Data in panels B and C are presented as the mean ± S.D. from at least three independent experiments.