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Copper assembly stabilizes CueO and results in decreased protease sensitivity. A, DSC analysis of apo-CueO (dotted line) and holo-CueO (solid line), indicating a shift of denaturation temperature upon cofactor assembly. B, limited proteolysis of apo-CueO and holo-CueO, showing that a number of trypsin-generated proteolysis products occurs only in the apo-CueO (indicated by asterisks).
