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Flexibility of folded apo-CueO domains likely promotes copper assembly. Structure of CueO using the coordinates of PDB file 1KV7 and the program Swiss-PDB viewer (50). A, type 2 and type 3 copper ions (black) connect domains in holo-CueO, resulting in a closed structure. B, the same structure without the copper ions visualizes the deep cleft between CueO domains, which in apo-CueO likely results in a domain flexibility (indicated by an arrow) that facilitates copper assembly.
