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. 2016 May 2;291(26):13546–13559. doi: 10.1074/jbc.M116.732693

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — ZnT2 mutants form homodimers based on BiFC analysis. MCF-7 cells were transiently co-transfected with YC- and YN-tagged ZnT2 plasmid pairs or with β2AR-YN as detailed below in the x axis. The percentage of transfected cells displaying YFP fluorescence (black bars) and the median YFP fluorescence (gray bar) were determined using flow cytometry and represent the fraction of ZnT-YC-YN positive cells. The negative controls consisted of co-transfection with ZnT2-YC and β2AR-YN. All p values (p < 0.05) revealed statistically significant differences when compared with the negative control of ZnT2-YC+β2AR-YN. All p values (p < 0.05) of the different mutants did not display any statistically significant differences when compared with WT-ZnT2-YC-YN. Error bars represent S.D.