FIGURE 2.

Subcellular localization of WT ZnT2 and mutant homodimers. MCF-7 cells were transiently co-transfected with the constructs described at the left side of the triplet panels. In addition, cells were co-transfected with the construct of the ER marker, the β subunit of signal recognition receptor tagged with CFP (SRβ-CFP), left panels. The green YFP fluorescence signal (YC-YN fluorescence) at the middle panels indicates dimer formation. Hoechst 33342 (blue fluorescence) was used to stain nuclei. Live MCF-7 cells were examined using confocal (LSM 710) fluorescence microscopy. A magnification of ×63 under immersion oil was used.