FIGURE 8.

Expression levels of the various Gly-280, Thr-312, and Glu-355 site-directed mutants of ZnT2 in DT40 ZnT1^−/−MT^−/−ZnT4^−/− cells. Confirmation of stable expression of the ZnT2-HA mutants in DT40 ZnT1^−/−MT^−/−ZnT4^−/− cells. Western blotting analysis was performed using anti-ZnT2 and β-tubulin antibodies. Aliquots (10 μg) of total cellular protein were loaded onto each lane, and the same membrane was used for detection of both ZnT2 and β-tubulin. β-Tubulin is shown to estimate the actual loading of the amount of proteins. − represents untransfected cells.