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Extensive examination of iPV neurons. A, the effect of cAMP analog of Bt2cAMP and 8-Br-cAMP on the conversion. B, dose-dependent manner of forskolin. C, exogenous Ascl1 expression was detected by RT-PCR. D, iPV neurons were checked by other specific neuronal markers of TH and HB9 and glial marker of GFAP. E, the conversion of adult tail-tip fibroblasts to neurons was examined by Tuj1 and PV staining. Scale bars: 50 μm.
