FIGURE 2.

Diabetes causes sustained impairment in factors of competency in culture. Measures of competence in CPCs isolated from WT and db/db mice are shown. A, traces of cell proliferation over a 48-h period. B, cell doubling time, calculated from data in A. C, cell survival, measured by lactate dehydrogenase assay, after a 16-h challenge with 500 μm H₂O₂. *, p ≤ 0.05; **, p ≤ 0.01 versus WT cells. D, relative expression of transcripts indicative of cardiovascular, fibroblastic, and preadipogenic gene programs. Each plot represents three independent experiments. *, p ≤ 0.05; **, p ≤ 0.01 versus WT undifferentiated. #, p ≤ 0.05; ##, p ≤ 0.01; ###, p ≤ 0.001 versus db/db undifferentiated. @@, p ≤ 0.01; @@@, p ≤ 0.001 versus WT differentiated.