FIGURE 5.
Effect of RR on single TREK channels. TREK-1-L, TREK-2-S, and TREK-2-S/TREK-1-L tandem channels were expressed in Xenopus oocytes. Single channel currents were recorded in symmetrical 140 mm K+ from outside-out membrane patches. Current traces displayed were filtered at 2 kHz. A–C, representative current recordings of outside-out membrane patches from Xenopus oocytes expressing TREK-1-L (A), TREK-2-S (B), and TREK-2-S/TREK-1-L tandem (C) at a membrane potential of −60 mV. Application of 30 μm RR is indicated by the horizontal bar on the recordings. The effect of RR was reversible. Channel activity (NPo) was determined from 30 to 60 s of recording before, during, and after application of RR. D, effect of 30 μm RR on channel activity of TREK-1-L (white circles, n = 4 patches), TREK-2-S (gray triangles, n = 7 patches), and TREK-2-S/TREK-1-L (black diamonds, n = 4 patches) channels is displayed as a scatterplot. The averages of the three groups are plotted as columns. The difference between the effect of RR on the channel activity of all three groups was found to be statistically significant (p < 0.05). * represents significant differences.