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Results of the esterification assays of phosphorylated 11-residue peptide (a) and paeninodin lasso peptide (b). Shown is the primary structure before and after the reaction (see black and red traces, respectively). Excerpts from 1H-31P heteronuclear multiple-bond correlation spectra of enzymatically produced (c) and synthetically derived (d) PadeA(13–23)-Ser-23-OPO32−, showing phosphoryl 31P-Ser-23 β-H cross-peaks.
