TABLE 1.
Proposed functions of ORFs in the putative lasso peptide biosynthetic gene clusters identified by genome mining in firmicutes
| Protein | Length (amino acids) | GenBankTM accession no. | Proposed function | Conserved domain analysis |
|---|---|---|---|---|
| PadeA | 42 | Precursor peptide | ||
| ThcoA | 42 | AGA59244.1 | ||
| BaceA | 41 | EJR65203.1 | ||
| PapoA | 42 | YP_008910696.1 | ||
| PadeB1 | 99 | WP_006678398.1 | Maturation enzyme | PqqD superfamily, |
| ThcoB1 | 98 | AGA59242.1 | B1 part of “split B” | Coenzyme PQQ synthesis protein D |
| BaceB1 | 101 | EJR65205.1 | Protease | |
| PapoB1 | 100 | YP_008910698.1 | ||
| PadeB2 | 142 | WP_006678399.1 | Maturation enzyme | Transglutaminase-like superfamily |
| ThcoB2 | 163 | AGA59241.1 | B2 part of split B | |
| BaceB2 | 157 | EJR65206.1 | Protease | |
| PapoB2 | 161 | YP_008910699.1 | ||
| PadeC | 649 | WP_006678396.1 | Maturation enzyme | Glutamine amidotransferases class-II (GATase) asparagine synthase B type |
| ThcoC | 645 | AGA59245.1 | Adenylation | |
| BaceC | 645 | EJR65202.1 | Cyclization | |
| PapoC | 641 | YP_008910695.1 | ||
| PadeD | 607 | WP_006678400.1 | Export | ABC-type multidrug transport system |
| ThcoD | 607 | AGA59240.1 | Immunity protein | |
| BaceD | 599 | EJR65208.1 | ||
| PapoD | 601 | YP_008910701.1 | ||
| PadeK | 313 | WP_006678397.1 | Putative kinase | HPrK-related kinase |
| ThcoK | 310 | AGA59243.1 | ||
| BaceK | 328 | EJR65204.1 | ||
| PapoK | 351 | YP_008910697.1 | ||
| BaceNT | 400 | EJR65207.1 | Putative nucleotidyltransferase | Uncharacterized nucleotidyltransferase |
| PapoNT | 506 | YP_008910700.1 | ||
| PapoST | 422 | YP_008910694.1 | Putative sulfotransferase | Sulfotransferase family |