FIGURE 3.

The O-glucose trisaccharide plays multiple roles in Notch-signaling activation and shows redundancy with monosaccharide O-fucose. A–L, SOPs in wing discs were detected by anti-Sens antibody staining (magenta). A–H, wing discs from a Notch knockdown (A), O-fut1^R245A homozygote (B), O-fut1^R245A and Uxs¹ double homozygote (C), O-fut1^R245A/O-fut1^R245A; Uxs/+ (D), shams homozygote (E), O-fut1^R245A and shams³⁴ double homozygote (F), rumi⁴⁴ homozygote (G), and O-fut1^R245A and rumi⁴⁴ double homozygote (H); the wing discs were isolated from larvae raised at 25 °C. I–L, wing discs from wild-type (I), O-fut1^R245A homozygote (J), shams homozygote (K), and rumi⁴⁴ homozygote (L) Drosophila; wing discs were isolated from third-instar larvae raised at 30 °C. Insets show magnified views of the areas in white squares, which contained SA-neuron precursors. The scale bar is 500 μm and applicable for all panels.