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Human lung fibroblasts were growth-arrested and were then stimulated with TGFβ1 (10 ng/ml) in the presence or absence of Si HC5. The RNA was collected after 72 h of incubation, and the effect of Si HC5 on copy number of heavy chain 5 (A) and the RQ of α-SMA (B) or TSG-6 (C) was determined by RT-qPCR. Data shown are mean and scatter of values from three independent experiments. *, p < 0.05; **, p < 0.005 for Si HC5 compared with scrambled.
