FIGURE 5.

Human lung fibroblasts were growth-arrested and were then stimulated with TGFβ1 (10 ng/ml). The RNA was collected after 72 h of treatment, and the RQ values of α-SMA (A, C, E, and G) and TSG-6 (B, D, F, and H) were determined by RT-qPCR. The panels show cells treated for 4 h before TGFβ1 stimulation with Si bikunin (A and B), cobalt chloride (0.05–1 mm) (C and D), oligo-HA (at 50 μg/ml) (E and F), anti-TGS-6 antibody A38 (G and H), and non-immune IgG control (50 μg/ml). Data shown are the mean and scatter of values in three independent experiments, each with more than two replicates. *, p < 0.05; **, p < 0.005 compared with scrambled (A and B) or TGFβ1-stimulated cells (C–H). Oligo 6 and Oligo 10, hyaluronan oligosaccharides (6 and 10 monosaccharide units, respectively).