FIGURE 2.

Double reciprocal plots of CaMKKα and β activities. Protein kinase activities of recombinant rat CaMKKα (closed circles, 8 μg/ml), rat CaMKKβ (open circles, 1 μg/ml) (A) and rat CaMKKα I322L mutant (closed triangles, 1 μg/ml, B) were measured as described in Fig. 1B with various concentrations of AMPK (1.9–7.7 μm) at 30 °C for 30 min in the presence of 200 μm [γ-³²P]ATP. The results represent duplicate experiments and are presented as double reciprocal plots (Lineweaver-Burk).