FIGURE 6.
Scaffold proteins differentially regulate the phosphorylation and localization of the aPKC substrate MARK2. A, immunoblots of COS-7 co-expressing mCherry-MARK2 (mCh-MARK2) and either vector, HA-PKCζ, CFP-IRS-1, CFP-PB1p62, or CFP-PB1Par6. The p595/total MARK2 ratios were quantified from five (PB1Par6), six (IRS-1), or seven (Vec, PKCζ, and PB1p62) independent experiments, normalized to Vec-transfected control, and plotted as means ± S.E. Statistical analysis was performed using separate t tests between each co-expression (PKCζ, IRS-1, PB1p62, and PB1Par6) and Vec control. Significance notated as ***, p < 0.001; **, p < 0.01; or n.s., not significant. B, images of live COS-7 expressing mCherry-MARK2 alone or co-expressed with YFP-tagged PKCζ, CFP-IRS-1, CFP-PB1p62, or CFP-PB1Par6.